Large Molecule LC (Bio LC)
The separation of active ingredients that have been manufactured using biological processes often requires a different approach.
HIC is an excellent technique for the separation of proteins without causing denaturing, therefore scaleable from analytical to downstream processing
SEC is used to separate components according to their molecular size. It is non-adsorbtive meaning no chemical interaction.
Ion exchange separates on the basis of charge on the molecule. It is used often for the separation of peptides, proteins and oligonucleotides
A 300Å pore size with a C4 or C8 phase is suitable for small proteins and large peptides. Proteins are retained by adsorbtion of the hydrophobic foot