HiScreen™ columns prepacked with Ni Sepharose™ 6 Fast Flow for method optimisation and parameter screening for purification of histidine-tagged proteins with Immobilised Metal ion Affinity Chromatography (IMAC).
- High binding capacity, approx. 40 mg/ml medium and negligible leakage of Ni²⁺
- Excellent choice for method optimisation and parameter screening due to the 10 cm bed height
- Easy connection in series to achieve 20 cm bed height
- Small bed volume gives fast results and minimal sample/buffer consumption
- Flow velocity: <450 cm/h
Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.