DNA modification enzymes供应商: Cytiva
GEHEE70092YEA 0 CNYGEHEE70092Y GEHEE70092Z GEHEE70092X
DNA modification enzymes
A high specific activity, heat-labile alkaline phosphatase. Completely and irreversibly inactivated in Tris buffers at pH 8,0 to 8,5 by heating for 15 minutes at 65 °C. No further treatment is necessary.
- Removes 5'-phosphates from DNA and RNA
- Easily inactivated by heat
Alkaline phosphatase is suitable for de phosphorylation of 5' and 3'-overhangs, recessed ends, blunt ends, and single nucleotides. Following incubation, illustra Alkaline Phosphatase can be easily inactivated by heating.
Use together with illustra Exonuclease I for cleaning up PCR reactions prior to downstream application such as DNA sequencing.
Unit Definition: One unit is the amount of the enzyme required to dephosphorylate 1 Â µg of linearized pUC57 DNA termini in 10 minutes at 37 °C.
Delivery information: Includes 10X reaction buffer: 200 mM Tris-HCl, pH 8,0, 100 mM MgCl₂; dilution buffer: 50 mM Tris-HCl, pH 8,0.