PNPP substrate供应商: Thermo Scientific
PIER37620EA 3375 CNYPIER37620 PIER34045 PIER37621
PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. The Pierce™ Phosphatase Substrate Kit contains PNPP tablets and a diethanolamine buffer to yield more than one litre of substrate. 1-Step PNPP is supplied pre-mixed with substrate buffer and is ready to use.
- One-component alkaline phosphatase (AP) substrate
- Absorbance maximum of 405 nm
When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.