PNPP substrate

供应商: Thermo Fisher Scientific
PIER34045 PIER37620 PIER37621
PNPP substrate
检测产品 ELISA试剂盒
PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. The Pierce™ Phosphatase Substrate Kit contains PNPP tablets and a diethanolamine buffer to yield more than one litre of substrate. 1-Step PNPP is supplied pre-mixed with substrate buffer and is ready to use.

  • One-component alkaline phosphatase (AP) substrate
  • Absorbance maximum of 405 nm

When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.

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