This antibody may cross-react with immunoglobulins from other species. Thermo Scientific Pierce Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Recommended Dilutions: Western Blot: 1:60-1:500, Immunohistochemistry: 1:6-1:60, ELISA: 1:60-1:500
Type: Secondary
Antigen: IgG
Clonality: Polyclonal
Clone:
Conjugation: HRP (Horseradish Peroxidase)
Epitope: Heavy and Light chain
Host: Goat
Isotype: IgG
Reactivity: Rabbit