The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. The Pierce™ Coomassie Plus Protein Assay is a ready-to-use, reducing agent-compatible, improved Bradford assay reagent to quickly measure (A595 nm) total protein concentration compared to a protein standard.
- Sensitive, fast and consistent protein assay kit
- Determine protein concentrations from 1 to 1500 μg/ml
- Ready-to-use dye binding reagent formulation
- Convenient microplate or cuvette format
- Compatible with reducing sugars, reducing substances and thiols
This modification of the well-known Bradford method greatly reduces the tendency of Coomassie reagents to give non linear response curves by a formulation that substantially improves linearity for a defined range of protein concentration. In addition, Coomassie Plus Reagent results in significantly less protein-to-protein variation than is observed with other Bradford-type Coomassie formulations.