LDS sample loading buffer, non reducing (4X)

VWRC1B1669-5ML VWRC1B1669-25ML
LDS sample loading buffer, non reducing (4X)
LDS sample loading buffer, non reducing (4X) is used to prepare protein samples for denaturing polyacrylamide gel electrophoresis using Bis-Tris and Laemmli gels.

  • Pre-mixed for convenience
  • Requires cold storage
  • 4X concentration allows more volume for protein per lane
  • Bring to room temperature prior to use

Mixing LDS sample loading buffer with protein samples only denatures the proteins without reducing them. Reducing agents such as DTT or 2-mercaptoethanol can be added to the buffer for protein sample preparation under reducing conditions. Glycerol is included in the loading buffer as a density agent to ensure the samples sink into the sample wells, while Coomassie® serves as a tracking dye to monitor the progression of electrophoresis.

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