Anti-CD3e Armenian Hamster Monoclonal Antibody (AP (Alkaline Phosphatase)C) [clone: 145-2C11]

供应商: eBioscience
EBIO17-0031-83EA 4300 CNY
EBIO17-0031-82 EBIO17-0031-63 EBIO17-0031-83 EBIO17-0031-81
Anti-CD3e Armenian Hamster Monoclonal Antibody (AP (Alkaline Phosphatase)C) [clone: 145-2C11]
抗体
Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells. Applications Reported: The 145-2C11 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Flow Cytometry: 0.25 µg/test

Type: Primary
Antigen: CD3e
Clonality: Monoclonal
Clone: 145-2C11
Conjugation: AP (Alkaline Phosphatase)C
Epitope:
Host: Armenian Hamster
Isotype: IgG
Reactivity: Mouse
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