Dye terminator removal, illustra™ AutoSeq™ G-50 columns

供应商: Cytiva
illustra™ AutoSeq™
GEHE27-5340-01EA 0 CNY
GEHE27-5340-01 GEHE27-5340-03 GEHE27-5340-02
Dye terminator removal, illustra™ AutoSeq™ G-50 columns
核酸试剂 核酸纯化试剂盒和试剂
illustra™ AutoSeq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high quality sequencing results since residual dye-terminators can obscure data. illustra™ AutoSeq™ G-50 consists of MicroSpin™ columns containing Sephadex™ G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

  • Columns are prepacked with Sephadex™ G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0,05% Kathon CG/ICP Biocide added as a preservative
  • Ready to use, enabling sample application to collection of purified product in less than 4 minutes

Effective purification is essential for high-quality sequencing results since residual dye-terminators can obscure data. Columns consist of MicroSpin columns containing Sephadex G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

Columns are prepacked with Sephadex G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative. Columns that are ready to use enable sample application to collection of purified product in less than 4 min.

订购须知 These illustra AutoSeq G-50 columns can be used to clean up reactions prepared with the BigDye Terminator v3.1 Cycle Sequencing Kit from Applied Biosystems.
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